The NMR service is equipped with five Avance Bruker spectrometers. Two spectrometers are self-service (300 MHz and 500 MHz) and two spectrometers (300 MHz and 500 MHz) operate in sample changer mode 24 hours a day. The flow of all automatic experiences that can be followed in real time by users via the Institute’s intranet. A 600 MHz is mainly intended for the analysis of natural products (high sensitivity / low quantity).
Operational Managers:
Jean-François Gallard (+33 1 69 82 31 25)
François Giraud (+33 1 69 82 37 64)
Scientific manager:
Ewen Lescop (+33 1 69 82 37 64)
RMN 300 MHz Sampler
Spectrometer with automatic sample changer
Equipement:
- QNP, BBFO probes
- Sample changer 60 samples
- Detection : 1H, 13C, 31P and 19F
Experiments carried out on the sample changer:
- 1D: 1H, 13C & DEPT 13C, 31P and 19F
- 2D: COSY, HMQC, HMBC
RMN 300 MHz “routine”
Spectrometer dedicated to 1D NMR experiments
Equipement:
- Dual C13 probe
Experiments doable as self-service:
- 1D : 1H, 13C, DEPT
RMN 500 MHz
Versatile spectrometer dedicated to 1D, 2D and inverse 2D NMR experiments, accessible by self-service via an internal reservation. It can also be used in automatic mode according to the needs of the service.
Equipement:
- BBI ATM probe
- TXI probe
- Gradient Z (50 G/cm)
- Three canals
- 60 sample changer
Experiments doable as self-service:
- 2D : HMQC, HMBC, HSQC, COSY, NOESY, ROESY
- 1D : 1H, 13C & DEPT 13C, 31P
RMN 500 MHz Changer
This spectrometer is intended for the analysis of samples in small quantities (± 1 mg) or unstable samples. It has a good sensitivity in direct carbon detection and allows us to realize in a short time all the 1D and 2D acquisitions needed to resolve organic structures automatically.
Equipement:
- BBO ATMA probe
- Gradient Z (50 G/cm)
- Two canals
- 60 sample changer
Experiments doable as self-service:
- 2D : HMQC, HMBC, HSQC, COSY, NOESY, ROESY …
- 1D : 1H, 13C & DEPT
RMN 600 MHz
The 600 MHz NMR spectrometer is a Bruker Avance spectrometer equipped with a TXI 1.7mm microprobe and a TBI probe (older and less sensitive). A variable temperature accessory BCU II allows to explore a temperature range depending on the possibilities of the probe used and the solvent used. This spectrometer solves difficult structural cases. The causes can be diverse:
- Low quantity of products,
- Complexity of the molecule,
- Balance problems requiring temperature variations
The TXI probe is a proton, carbon, nitrogen reverse probe for 15N studies. By varying the temperature and solvent variations, the protonation sites can be highlighted. It is necessary to have previously recorded a spectrum on a routine spectrometer to obtain a study on this 600 MHz.
Mode of operation of the service
The service has a powerful real-time booking tool that optimizes the use of the spectrometer. The flow of all automatic experiences that can be followed in real time by users via the Institute’s intranet. The NMR data is stored in real time on a shared storage space, secure of very large capacity. NMR data is processed directly on the users’ workstations. The service participated in the beta test phase for the software NMRnotebook which has since been used by the Institute’s chemists for the processing and visualization of NMR spectra.
