RMNHC@UPSay Platform
Contact : rmnhc-upsay@cnrs.fr
Scientific manager: Carine van Heijenoort (+33 1 69 82 37 94)
Operationnal manager: François Giraud (+33 1 69 82 37 64)
Secretariat-management: Alda da Costa (+33 1 69 82 36 71)
National Research Infrastructure Infranalytics (950 MHz)
Scientific manager: Ewen Lescop (+33 1 69 82 37 64)
Operational manager: Nelly Morellet (+33 1 69 82 37 62)
Communication correspondent: François Giraud (+33 1 69 82 37 64)
Secretariat-management: Alda da Costa (+33 1 69 82 36 71)
Savoir-faire
High field NMR spectrometers are managed by the ‘NMR, Biologiy and Structural Chemistry’ team of the Institute of Chemistry of Natural Substances (ICSN), CNRS, Gif/Yvette.
Design of NMR structural study projects adapted to the systems and questions asked and support during the project stages.
- Main fields of application:
- Mainly liquid NMR but also NMR of soft and solid matter.
- Structure, dynamics and interactions of biological macromolecules: large proteins, intrinsically disordered proteins, structural changes, multiple states, post-translational modifications, protein/nucleic acid/ligand interactions.
- Natural substances, complex mixtures, small molecules/membranes, liposomes, micelles, fibers, resins.
- Expertise in NMR methodology:
- Fast NMR (BEST, SOFAST…), Non-linear sampling or projection methods, 3D, 4D triple resonance experiments for proteins and nucleic acids, Relaxation and Relaxation Dispersion, Residual Dipolar Couplings, Methyl NMR, Protein-Ligand Interactions, DOSY, high pressure.
- Development of pulse sequences.
- Data analysis and interpretation:
- Software programming, calculations (MATLab, Python, HADDOCK, CYANA, ARIA)
- Production of protein samples for NMR / Cell culture
- Expertise in the production of recombinant proteins labelled with isotopes (15N/13C/2H) in bacteria and insect cells. Protocols available on request.