CV A. Chevalier

CNRS researcher CRCN, Institut de Chimie des Substances Naturelles, UPR-CNRS 2301, Gif-Sur-Yvette, France.
2017-2018 Post-doctoral Fellow at CEA Saclay (Saclay, France, Dr Frédéric Taran).
2016-2017 Post-doctoral Fellow at Department of Bioorganic Chemistry (Rouen, France, Dr Xavier Franck).
2014-2016 Post-doctoral Fellow at Arizona State University (US, Tempe AZ, with Pr Sidney M. Hecht).
2011-2014 PhD at Department of Bioorganic Chemistry (UMR-CNRS 6014, Rouen, France) under the supervizion of Prof. Pierre-Yves Renard and Prof. Anthony Romieu

Synthesis and functionalization of organic fluorophores

Smart fluorogenic probes as sensors for enzymatic activities

Self immolative linkers

Drug delivering

32 publications – 4 patents – H-Index : 13

7 oral communications and conferences, 3 poster communications

  1. Chevalier, A., Naphthalimide/heterocycles fused hybrid dyes, how and why ? An overview of the latest developments in the quest for dyes with innovative optical properties. Org. Biomol. Chem. 2023, DOI: 10.1039/d3ob01035j Accepted.
  2. Tacke, E.; Hoang, M.-D.; Tatoueix, K.; Keromnes, B.; Van Eslande, E.; Durand, P.; Pieters, G.; Chevalier, A., Unprecedented perspectives on the application of CinNapht fluorophores provided by a ′′late-stage′′ functionalization strategy. Chem. Sci. 2023, 14 (22), 6000-6010, 10.1039/d3sc01365k.
  3. Riberaud, M.; Porte, K.; Chevalier, A.; Madegard, L.; Rachet, A.; Delaunay-Moisan, A.; Vinchon, F.; Thuery, P.; Chiappetta, G.; Champagne, P. A.; Pieters, G.; Audisio, D.; Taran, F., Fast and Bioorthogonal Release of Isocyanates in Living Cells from Iminosydnones and Cycloalkynes. J. Am. Chem. Soc. 2023, 145 (4), 2219-2229, 10.1021/jacs.2c09865.
  4. Hoang, M.-D.; Savina, F.; Durand, P.; Meallet-Renault, P. R.; Clavier, G.; Chevalier, A., Tunable Naphthalimide/Cinnoline-Fused (CinNapht) Hybrid Dyes for Fluorescence Imaging in Living Cells. ChemPhotoChem 2022, 6 (11), e202200138, 10.1002/cptc.202200138.
  5. de Saint Germain, A.; Clavé, G.; Schouveiler, P.; Pillot, J.-P.; Singh, A.-V.; Chevalier, A.; Daignan Fornier, S.; Guillory, A.; Bonhomme, S.; Rameau, C.; Boyer, F.-D., Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application. Front. Plant. Sci. 2022, 13, 887347, 10.3389/fpls.2022.887347.
  6. Boujut, M.; Chevalier, A.; Schapman, D.; Benard, M.; Galas, L.; Gallavardin, T.; Franck, X., Indazole versus indole-based cationic merocyanines with red shifted in-cellulo emission for selective mitochondria imaging. Dyes Pigm. 2022, 198, 109988, 10.1016/j.dyepig.2021.109988.
  7. Renault, K.; Chevalier, A.; Bignon, J.; Jacquemin, D.; Richard, J.-A.; Romieu, A., Coumarin-Pyronin Hybrid Dyes: Synthesis, Fluorescence Properties and Theoretical Calculations. ChemPhotoChem 2021, 5 (9), 822-838, 10.1002/cptc.202100069.
  8. Hoang, M.-D.; Bodin, J.-B.; Savina, F.; Steinmetz, V.; Bignon, J.; Durand, P.; Clavier, G.; Meallet-Renault, R.; Chevalier, A., « CinNapht » dyes: a new cinnoline/naphthalimide fused hybrid fluorophore. Synthesis, photo-physical study and use for bio-imaging. RSC Adv. 2021, 11 (48), 30088-30092, 10.1039/d1ra05110e.
  9. Dejouy, G.; Renault, K.; Bonnin, Q.; Chevalier, A.; Michaudet, C.; Picquet, M.; Valverde, I. E.; Romieu, A., Fluorogenic Enzyme-Triggered Domino Reactions Producing Quinoxalin-2(1H)-one-based Heterocycles. Org. Lett. 2020, 22 (16), 6494-6499, 10.1021/acs.orglett.0c02287.
  10. Del Vecchio, A.; Talbot, A.; Caille, F.; Chevalier, A.; Sallustrau, A.; Loreau, O.; Destro, G.; Taran, F.; Audisio, D., Carbon isotope labeling of carbamates by late-stage [11C], [13C] and [14C]carbon dioxide incorporation. Chem. Commun. 2020, 56 (78), 11677-11680, 10.1039/d0cc05031h.
  11. Del Vecchio, A.; Caille, F.; Chevalier, A.; Loreau, O.; Horkka, K.; Halldin, C.; Schou, M.; Camus, N.; Kessler, P.; Kuhnast, B.; Taran, F.; Audisio, D., Late-Stage Isotopic Carbon Labeling of Pharmaceutically Relevant Cyclic Ureas Directly from CO2. Angew. Chem., Int. Ed. 2018, 57 (31), 9744-9748, 10.1002/anie.201804838.
  12. Chevalier, A.; Ouahrouch, A.; Arnaud, A.; Gallavardin, T.; Franck, X., An optimized procedure for direct access to 1H-indazole-3-carboxaldehyde derivatives by nitrosation of indoles. RSC Adv. 2018, 8 (24), 13121-13128, 10.1039/c8ra01546e.
  13. Zhang, Y.; Chevalier, A.; Khdour, O. M.; Soto, L. M.; Hecht, S. M., Inhibition of Human Cancer Cell Growth by Analogues of Antimycin A. Planta Med. 2017, 83 (18), 1377-1383, 10.1055/s-0043-112343.
  14. Vivoli, M.; Renou, J.; Chevalier, A.; Norville, I. H.; Diaz, S.; Juli, C.; Atkins, H.; Holzgrabe, U.; Renard, P.-Y.; Sarkar-Tyson, M.; Harmer, N. J., A miniaturized peptidyl-prolyl isomerase enzyme assay. Anal. Biochem. 2017, 536, 59-68, 10.1016/j.ab.2017.08.004.
  15. Makrerougras, M.; Coffinier, R.; Oger, S.; Chevalier, A.; Sabot, C.; Franck, X., Total Synthesis and Structural Revision of Chaetoviridins A. Org. Lett. 2017, 19 (15), 4146-4149, 10.1021/acs.orglett.7b02053.
  16. Chevalier, A.; Renard, P.-Y.; Romieu, A., Azo-Based Fluorogenic Probes for Biosensing and Bioimaging: Recent Advances and Upcoming Challenges. Chem. – Asian J. 2017, 12 (16), 2008-2028, 10.1002/asia.201700682.
  17. Chevalier, A.; Khdour, O. M.; Schmierer, M.; Bandyopadhyay, I.; Hecht, S. M., Influence of substituent heteroatoms on the cytoprotective properties of pyrimidinol antioxidants. Bioorg. Med. Chem. 2017, 25 (5), 1703-1716, 10.1016/j.bmc.2017.01.030.
  18. Chevalier, A.; Zhang, Y.; Khdour, O. M.; Kaye, J. B.; Hecht, S. M., Mitochondrial Nitroreductase Activity Enables Selective Imaging and Therapeutic Targeting. J. Am. Chem. Soc. 2016, 138 (37), 12009-12012, 10.1021/jacs.6b06229.
  19. Chevalier, A.; Zhang, Y.; Khdour, O. M.; Hecht, S. M., Selective Functionalization of Antimycin A Through an N-Transacylation Reaction. Org. Lett. 2016, 18 (10), 2395-2398, 10.1021/acs.orglett.6b00882.
  20. Chevalier, A.; Alam, M. P.; Khdour, O. M.; Schmierer, M.; Arce, P. M.; Cripe, C. D.; Hecht, S. M., Optimization of pyrimidinol antioxidants as mitochondrial protective agents: ATP production and metabolic stability. Bioorg. Med. Chem. 2016, 24 (21), 5206-5220, 10.1016/j.bmc.2016.08.039.
  21. Roubinet, B.; Chevalier, A.; Renard, P.-Y.; Romieu, A., A Synthetic Route to 3-(Heteroaryl)-7-hydroxycoumarins Designed for Biosensing Applications. Eur. J. Org. Chem. 2015, 2015 (1), 166-182, 10.1002/ejoc.201403215.
  22. Chevalier, A.; Renault, K.; Boschetti, F.; Renard, P.-Y.; Romieu, A., Rapid Synthesis of Unsymmetrical Sulforhodamines Through Nucleophilic Amination of a Monobrominated Sulfoxanthene Dye. Eur. J. Org. Chem. 2015, 2015 (1), 152-165, 10.1002/ejoc.201403165.
  23. Chevalier, A.; Piao, W.; Hanaoka, K.; Nagano, T.; Renard, P.-Y.; Romieu, A., Azobenzene-caged sulforhodamine dyes: a novel class of ′turn-on′ reactive probes for hypoxic tumor cell imaging. Methods Appl. Fluoresc. 2015, 3 (4), 044004/1, 10.1088/2050-6120/3/4/044004.
  24. Jouanno, L.-A.; Chevalier, A.; Sekkat, N.; Perzo, N.; Castel, H.; Romieu, A.; Lange, N.; Sabot, C.; Renard, P.-Y., Kondrat’eva Ligation: Diels-Alder-Based Irreversible Reaction for Bioconjugation. J. Org. Chem. 2014, 79 (21), 10353-10366, 10.1021/jo501972m.
  25. Chevalier, A.; Renard, P.-Y.; Romieu, A., Straightforward Access to Water-Soluble Unsymmetrical Sulfoxanthene Dyes: Application to the Preparation of Far-Red Fluorescent Dyes with Large Stokes’ Shifts. Chem. Eur. J. 2014, 20 (27), 8330-8337, 10.1002/chem.201402306.
  26. Chevalier, A.; Renard, P.-Y.; Romieu, A., Azo-Sulforhodamine Dyes: A Novel Class of Broad Spectrum Dark Quenchers. Org. Lett. 2014, 16 (15), 3946-3949, 10.1021/ol501753b.
  27. Chevalier, A.; Renard, P.-Y.; Romieu, A., Straightforward synthesis of bioconjugatable azo dyes. Part 1: Black Hole Quencher-1 (BHQ-1) scaffold. Tetrahedron Lett. 2014, 55 (50), 6759-6763, 10.1016/j.tetlet.2014.10.053.
  28. Chevalier, A.; Renard, P.-Y.; Romieu, A., Straightforward synthesis of bioconjugatable azo dyes. Part 2: Black Hole Quencher-2 (BHQ-2) and BlackBerry Quencher 650 (BBQ-650) scaffolds. Tetrahedron Lett. 2014, 55 (50), 6764-6768, 10.1016/j.tetlet.2014.10.054.
  29. Chevalier, A.; Mercier, C.; Saurel, L.; Orenga, S.; Renard, P.-Y.; Romieu, A., The first latent green fluorophores for the detection of azoreductase activity in bacterial cultures. Chem. Commun. 2013, 49 (78), 8815-8817, 10.1039/c3cc44798g.
  30. Chevalier, A.; Massif, C.; Renard, P.-Y.; Romieu, A., Bioconjugatable Azo-Based Dark-Quencher Dyes: Synthesis and Application to Protease-Activatable Far-Red Fluorescent Probes. Chem. Eur. J. 2013, 19 (5), 1686-1699, 10.1002/chem.201203427.
  31. Chevalier, A.; Hardouin, J.; Renard, P.-Y.; Romieu, A., Universal Dark Quencher Based on « Clicked » Spectrally Distinct Azo Dyes. Org. Lett. 2013, 15 (23), 6082-6085, 10.1021/ol402972y.
  32. Chevalier, A.; Dubois, M.; Le Joncour, V.; Dautrey, S.; Lecointre, C.; Romieu, A.; Renard, P.-Y.; Castel, H.; Sabot, C., Synthesis, Biological Evaluation, and in Vivo Imaging of the first Camptothecin-Fluorescein Conjugate. Bioconjugate Chem. 2013, 24 (7), 1119-1133, 10.1021/bc3005304.

Dr. Gilles CLAVIER (ENS Paris/Saclay, UMR 8531, Orsay)

Pr. Rachel MEALLET-RENAULT (ISMO, UMR 8214, Orsay)

Dr Gregory PIETERS (CEA Saclay)

Dr Anthony ROMIEU (ICMUB, UMR 6302, Dijon)

ANR – PEC – SydFluo (ANR): 2024-2028

CHARM3AT Labex (ANR-11-rDEX-0003-02-RD280): 2022-2024

ANR – JCJC – MitoRedAct (ANR-21-CE18-0005-01): 2022-2025

Fondation ARC (ARCPJA12020060002151): 2021-2023

CHARM3AT Labex (ANR-11-rDEX-0003-02-RD207): 2020-2021


  • Member of conferences organization committee of ICSN
  • Member of the redaction committee for Twitter account of ICSN.
  • Member of Student Seminar committee of ICSN
  • Webmaster of Chemical Biology department for ICSN website

CNRS Research Associate 


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